To All:
All email packages(clients) give the option to send text only messages. 
  Histonet as is pointed out below is text only because it is used 
worldwide and the html puts a huge load on the second and third world 
which in some places must use very slow telephone connections to 
retrieve the mail (2400 Baud would not be unusual!).
I have periodically made the appeal for people to use the plan text 
mode and not the "fancy" but unreadable html encoding for email.
It is really simple to use text only and avoid the garbage in the 
emails....
Thanks again for your support of Histonet...
Herb Hagler

On Sunday, Nov 17, 2002, at 23:58 US/Central, HistoNet Server wrote:

>
> ----------------------------------------------------------------------
>
> Date: 17 Nov 2002 05:30:12 -0600
> From: Susan Owens 
> Subject: Computer lingo
>
>> On another note-what's with the garbage coming in with posts-I had to 
>> scan
>> through most of today's digest to get past all these nonsense letters 
>> and
>> numbers?  Is there anyway the Digest could be sent without all the 
>> computer
>> lingo and additional stuff?  Just an idea!
>
>> Thanks.
>> Sue Becker, HTL
>> Albany, NY 12205
> ******************
>
> Sue, I have been getting the 'digest' for several years now and your
> questions keeps coming up...
> The answer is YES. I'm not a computer person, but my understanding is 
> it is
> caused because  'html' does not do well with all computers...MOST list
> serves like the Histonet want mail sent to them in 'plain text', NOT 
> html
> text.....
> In my address book(Outlook Express), when I input a 'list serve'
> address(like Histonet) I check the box to send all mail to this 
> address as
> 'plain text'.....Either some people don't do this or maybe their 
> computer
> doesn't give them a choice....The result is all that crazy, unreadable
> mess.....And for those of us who get the 'digest' it can give one a 
> headache
> trying to scan through it all.
>
>
> Susan Owens-TX
> ohenry@dfw.net
> voice: 817-261-7938
> fax: 817-548-9876
>
> "A bad day at the dog show is better then a good day at work!"
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 17 Nov 2002 06:15:29 -0600
> From: Lee & Peggy Wenk 
> Subject: Re: Spirochete controls?
>
> Three options I can think of:
>
> 1. MAKE THEIR OWN
>
> Take fresh unfixed lung (slightly edematous is better) or fresh 
> placenta
> (with most of the blood squeezed/blotted out), and cut it into 3-5 mm 
> cubes.
>
> Take the pieces down to microbiology. Have them make a fresh broth of 
> liquid
> culture media and non-pathogenic spirochete in a large test 
> tube/centrifuge
> tube. (Micro will know which culture media is best to use for the
> spirochete.) Put tissue pieces into the culture media/spirochete 
> mixture.
> Cover.
>
> Incubate in 37 degree C. oven overnight to 24 hours.
>
> Add fixative, and allow to fix for 30 minutes. Then pour out fixative 
> and
> culture media, and add fresh fixative. (The first fixative is diluted 
> by the
> culture media, which is why a 2nd "dose" is needed. Also, the culture 
> media
> has "bugs" in it, so it is better to fix the microorganisms, before 
> dumping
> it down the sink or wherever you disposal of it.)
>
> Allow to fix overnight, then place in cassettes, process and embed as 
> usual.
>
> There won't be any inflammatory reaction in the tissue, but there will 
> be
> micro-organisms in the tissue, and both spirochetes and tissues will 
> have
> been fixed and processed as that lab's regular tissues.
>
> P.S. This technique also works for gram + and - bacteria, AFB and 
> fungus.
>
> 2. PLACENTA:
>
> In some parts of the country, there is an increase in syphilis. There 
> is
> also an increase in women who are pregnant being infected.
>
> Those who are in these areas, please have your pathologists notify you 
> when
> they receive a placenta with syphilis.
>
> Then process the ENTIRE placenta. You can put several LARGE pieces in 
> each
> cassette, and process all of them.
>
> Then, embed some of the block, after cutting the pieces to a more 
> manageable
> size and embedding them into more cassettes. (In other words, if the 
> tissue
> is 1.5 cm x 1.5 cm by 3 mm, use a razor blade or scalpel at the 
> embedding
> station, and trim it into 5 mm x 5 mm pieces, and embed each of these 
> pieces
> into a separate cassette. By my calculations, you should be able to 
> get 9
> more cassettes from this one piece of tissue.)
>
> This saves on number of cassettes going through the tissue processor. 
> Also,
> you can then embed the "cut down" smaller pieces into just the bottoms 
> of
> the cassettes. That way, you can save money by using the "bottoms only"
> cassettes and/or not having to clean the metal tops. Also, you will 
> have few
> cassettes to store, if you don't embed them all at that time (see next
> paragraph).
>
> The rest of the processed cassettes that you don't want to embed right 
> now,
> place 8 in a slide box (yes, 8 will fit in 2 layers of 4 cassettes 
> each),
> pour more paraffin into the box to cover the cassettes. Add a paper 
> label on
> top of the hardening paraffin as to what the tissue control is. Then 
> write
> the same information on the lid of the box, and store in a cupboard, 
> until
> you need more. Don't put on the bottom shelf of the cupboard, if there 
> is a
> light under the cupboard. Can melt the paraffin (been there, done 
> that),
>
> Of course, then contact Histonet, and be willing to share with the 
> rest of
> us.
>
> 3. ANIMAL LEPTOSPIRA
>
> Become friends with your local veterinary lab.
>
> If they get any cases of Leptospira in - have them contact you. There 
> are
> different Leptospira that can infect rodents, dogs, pigs, cattle and
> raccoons. (Weil's disease is a jaundice or meningitis that occurs in 
> humans
> who are exposed to the urine of these infected animals. In the US, I 
> think
> the most common source is from dogs.)
>
> Once you get a kidney from an infected animal, fix, process, and 
> proceed the
> same as #2.
>
> Remember to share with Histonet, of course.
>
> Good luck to all.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
>
>
> - ----- Original Message -----
> From: "Morken, Tim" 
> To: "'Histonet'" 
> Sent: Friday, November 15, 2002 9:54 AM
> Subject: Spirochete controls?
>
>
>> Does anyone know a good source for spirochete controls? (we at CDC 
>> have
> our
>> own but don't give them out for controls. I am asking for someone who
> needs
>> controls but can't find a source).
>>
>> Tim Morken
>> CDC, Atlanta
>>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 17 Nov 2002 11:00:42 -0600
> From: Barbara Stancel 
> Subject: Re: Recyclers
>
> Dear Mary,
>
> We have been recycling xylene and alcohol for almost 12 years. 
> Previously we
> had two excellent glass distillation units and we were very hesitant 
> about
> replacing them. About 18 months ago, through special hazardous waste
> reduction money, we had the opportunity to order new recyclers. We 
> demo-ed
> or traveled to other labs to see and hear about the new enclosed 
> recycling
> units.
>
> The two units we were considering were equal on almost all of their 
> features
> and ability to recycle many solvents. Both companies have good customer
> service and good products. Of the two which made the last round, we 
> chose
> the CBG (800-941-9484) for two reasons. Mobility-a feature we liked 
> because
> we could roll them out in the hall and get the floors under them
> cleaned/waxed. Second, because they were very, very quiet!
>
> Good luck in your decision making. You have made a good start by 
> checking
> with the HISTONET. Please call if there are any specific questions you 
> may
> have about our choice.
>
> Histologically yours,
> Barbara H. Stancel, HTL(ASCP)HT
> USDA, FSIS, OPHS, Eastern Laboratory, Pathology
> RRC, 950 College Station Road
> Athens, Georgia  30604
> phone: (706) 546-3556
> fax: (706) 546-3589
>
>
> Message:
> I know there's several good brands of recyclers on the Market, but 
> before I
> start trialing some, I ask opinions of those of you on the histonet 
> that
> recycle.Reagents to possible recycle include alcohol, xylene, clearite,
> Penfix and formalin.Any opinions from our experts would be appreciated.
> Thanks
>
> Mary McCoy
> Pathology Coordinator
> Lakeland Regional Health System
> St. Joseph, MI
>
>
> _________________________________________________________________
> Add photos to your messages with MSN 8. Get 2 months FREE*.
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>
>
>
> ----------------------------------------------------------------------
>
> Date: 17 Nov 2002 15:45:28 -0600
> From: zenobia haffajee 
> Subject: Re: MLH1 antibody
>
> Hi Steven
>
> what have you tried when optimising the MLH1 antibody?  What is the 
> source
> of your MLH1 antibody.
> We use the one from Pharmingen. Works well with microwave antigen 
> retrival
> in citrate buffer. We do incubate our slides overnight though. hope 
> this
> helps.
>
> Zenobia Haffajee
> AIP laboratories
> Australia
>
>
> - ----- Original Message -----
> From: 
> To: 
> Sent: Sunday, November 10, 2002 1:01 AM
> Subject: MLH1 antibody
>
>
>>
>> Still looking for some help with the MLH1 antibody. Is anyone doing 
>> the
>> stain at the moment and if yes .. how are you getting it to work??!
>>
>> any help would be great
>>
>> Stephen
>>
>>
>>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 17 Nov 2002 18:30:52 -0600
> From: "Michelle Mcanulty-Smith - NeuronZ Ltd."
> 
> Subject: AP and APP-IV
>
> Hi histo folk,
>
> I have a query from a student who is gathering information for 
> staining both
> arterial and venular portions of a capillary.
>
> He has some info on Alkaline phosphatase and Dipeptidyl peptidase IV 
> which
> will apparently demonstrate both the portions of the capillary.
>
> Has anyone got experience with this method that they might like to 
> share?
> All suggestions welcome.
>
> Thanks in advance
>
> Michelle.
>
> Michelle McAnulty-Smith
> Histology Co-ordinator
>
> NeuronZ Ltd
> Auckland
> New Zealand
> Tel: 09 367 7167 ext. 7060
> Fax: 09 367 7186
>
> E-mail:  michelle.mcanulty-smith@neuronz.com
>
>
> ######################################################################
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>
> Here are the messages received yesterday!
>