Many, many years ago, in my first histology job, I sectioned frozen thoraxes
of domestic houseflies (pesticide localization studies). The hardest part
was getting the little buggers oriented! I practiced on about 20 or 30
before finding a technique that worked for me. I kept the tissue on dry ice
and the cryostat temperature at about -20. I don't remember problems
sectioning through the frozen cuticle. And best I can remember, I serial
sectioned each thorax putting all of the sections from one fly on a big ole
glass 3"x 3" slide(no frosted end).
My advice: practice with some spare mosquitoes and vary your temperature
until your technique is comfortable for you and satisfies your researcher.
Best of luck!
Barbara H. Stancel, HTL(ASCP)HT
USDA, FSIS, OPHS, Eastern Laboratory, Pathology
RRC, 950 College Station Road
Athens, Georgia 30604
phone: (706) 546-3556
fax: (706) 546-3589
From: "Salcedo, Rudy"
Subject: FW: mosquitoes
Date: Thu, 07 Nov 2002 08:13:07 -0600
I have an investigator that would like for me to section frozen mosquitoes
any suggestions would be appreciated.
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