eNOS in eyes and Sihler stain

From:Cheryl Crowder

Good morning - I saw you question on the Histonet about a stain for nerve
branching.  I have found the directions and will send them to you by 
personal e-mail. 
     Now, could you help me with my problem?  I am starting a research 
project using eNOS I, II, III.  The primary Abs are from Serotec and 
their help is minimal.
    Has anyonedone this type of IHC and what procedure did you use?  The 
scant info I got did not mention antigen retrival and had a broad area 
for dilutions.  Any help you can give me would "make my day".  Thank 
you.  Cheryl


Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA #160#70803

(225) 578-9784
FAX #160#(225) 578-9720




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