anti-betagalactosidase in paraffin slides

From:mrl0627@mail.ecu.edu


Hello:
Has anyone had success with antibodies against B Gal in paraffin embedded tissue?  We have tried several with no success and ICN does not recomment the Cappel rabbit ab in paraffin.  I cannot use frozen sections (am working with embryonic-early post-natal mouse inner ear and need sharp morphology.  Is there hope?  Thanks in advance for any assistance.
M.Loomer @ East Carolina University 
								
			



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