Squamous epithelial cells in lung tissue.
Dear All,
For the last twenty years we have been using Harold
Attwood's Method for Amniotic Fluid Embolism. Now we find that Alcian
green is unavailable. In the past we were able to make do with a mix of
Alcian Blue and Alcian Yellow at pH 2.5. Now we find that mixing these two
dyes simply causes them to form a gel like mixture which does not stain (almost
colloidal).
Does anyone have any idea of where I might be able
to obtain Alcian Green or a dye we can use as a substitute in this
stain.
Alcian Blue or Alcian Yellow alone is
too strong a colour to allow the squamous cells to be seen as they are usually
very pale pink.
Any advice would be appreciated.
Many thanks
Bill Sinai
Laboratory Manager
Tissue
Pathology
ICPMR
P.O. Box 533
Wentworthville NSW 2145
Ph 02 9845
7774
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