Re: paraffin
"DiCarlo, Margaret" wrote:
> ... Can anyone recommend a paraffin that would
> make cutting bone sections easier?
Some years ago Russ Allison did comparative studies
of a large number of paraffin formulations. One of his
conclusion was that none were any better than the
others. Here are some of the publications:
Allison RT (1978) The crystaline nature of histology waxes:
a preliminary communication. Medical Laboratory Sciences
35: 355-363.
Allison RT (1979) The crystaline nature of histology waxes:
the effects of microtomy on the micro-structure of paraffin
wax in sections. Medical Laboratory Sciences 36: 359-372.
Allison RT, Lloyd D (1996) Measuring infiltration during
paraffin wax processing for histology. British Journal of
Biomedical Science 53: 235-237.
Allison RT, Bryant D (1998) Effects of processing at 45C on
staining. Biotechnic & Histochemistry 73: 128-136.
For more informal information there are various Histonet
communications from Russ Allison. Try www.histosearch.com.
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/
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