Re: paraffin

From:"J. A. Kiernan"

"DiCarlo, Margaret" wrote:
> ... Can anyone recommend a paraffin  that would 
> make cutting bone sections easier?

Some years ago Russ Allison did comparative studies
of a large number of paraffin formulations. One of his
conclusion was that none were any better than the
others. Here are some of the publications:

Allison RT (1978) The crystaline nature of histology waxes: 
a preliminary communication. Medical Laboratory Sciences 
35: 355-363.

Allison RT (1979) The crystaline nature of histology waxes: 
the effects of microtomy on the micro-structure of paraffin 
wax in sections. Medical Laboratory Sciences 36: 359-372.

Allison RT, Lloyd D (1996) Measuring infiltration during 
paraffin wax processing for histology. British Journal of 
Biomedical Science 53: 235-237.

Allison RT, Bryant D (1998) Effects of processing at 45C on 
staining. Biotechnic & Histochemistry 73: 128-136.

For more informal information there are various Histonet
communications from Russ Allison. Try www.histosearch.com.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/



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