Re: paraffin

From:"J. A. Kiernan"

"DiCarlo, Margaret" wrote:
> ... Can anyone recommend a paraffin  that would 
> make cutting bone sections easier?

Some years ago Russ Allison did comparative studies
of a large number of paraffin formulations. One of his
conclusion was that none were any better than the
others. Here are some of the publications:

Allison RT (1978) The crystaline nature of histology waxes: 
a preliminary communication. Medical Laboratory Sciences 
35: 355-363.

Allison RT (1979) The crystaline nature of histology waxes: 
the effects of microtomy on the micro-structure of paraffin 
wax in sections. Medical Laboratory Sciences 36: 359-372.

Allison RT, Lloyd D (1996) Measuring infiltration during 
paraffin wax processing for histology. British Journal of 
Biomedical Science 53: 235-237.

Allison RT, Bryant D (1998) Effects of processing at 45C on 
staining. Biotechnic & Histochemistry 73: 128-136.

For more informal information there are various Histonet
communications from Russ Allison. Try
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

<< Previous Message | Next Message >>