Re: in situ


Here's an excellent web site for ISH protocols:
----- Original Message -----
From: Robbin Newlin
To: HistoNet Server
Sent: Wednesday, October 30, 2002 11:17 AM
Subject: in situ

Hi All
I run a core facility and we service approx. 500 post docs and grad students. The powers that be have decided that we should offer in situ as a core service. I have my own list of reservations as to why this is not practical, but I must keep an open mind, is anyone out there doing this, if so,  how do you charge back, who makes the probe, who is responsible for optimizing the probe, do you use radiolabeled probes or not. I also would like to know if there are any seminars/classes being held since I have not done this for almost 8 years I feel the need for a refresher course. One more thought, this will primarily be used in conjunction with our DNA Microarray facility. Thanks in advance for your time.
Robbin Newlin, HT,QIHC ASCP
Manager Histology Core Facility
The Burnham Institute
10901 North Torrey Pines Road
La Jolla, CA. 92037
(858) 646-3100 Ext. 3552

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