Re: Whole mount embryo stain

From:Geoff McAuliffe

    Under "Special Proceedures" in Humason's "Animal Tissue Techniques" there are several pages devoted to chick embryos, fixing, staining of whole mounts, etc. Perhaps someone on the list has practical experience.


"Johnson, Teri" wrote:

> Is anybody doing any sort of embryo staining which allows for easy visualization of somites?  We're looking for a technique (not ISH or IHC) that will allow for easier counting of somites (in chick), hopefully that is compatible with prior or subsequent ISH techniques.  Does such a thing exist for whole embryos?
> Thanks!
> Teri Johnson
> Managing Director Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri  64110

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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