Forget ORO.  Osmicate and process either into paraffin or into resin.

Alex K

At 15:41 13/11/02 -0800, Trenton R. Schoeb wrote:
>I'd appreciate hearing from anyone who's had problems with Oil Red O 
>staining of frozen sections of formalin fixed liver and has found 
>solutions. Basic problem is sections not adhering to slide and blobs of fat 
>coalescing on slide and spreading under sections.
>
>--------------------------------------------------
>Trenton R. Schoeb, DVM, PhD, Diplomate ACVP
>Professor, Department of Genetics
>University of Alabama at Birmingham
>trs@uab.edu
>205-934-2288
>Department office: 205-934-2117
>Fax: 205-975-4418
>http://main.uab.edu/show.asp?durki=34925
>
>US mail:                    Fed Ex:
>VH 402                      402 Volker Hall
>1530 3rd Ave. S.            1670 University Blvd.
>Birmingham, AL 35294-0019   Birmingham, AL 35294
>--------------------------------------------------
>
>
>
Alex Knisely, MD
Consultant Histopathologist

alex.knisely@kcl.ac.uk
 
Institute of Liver Studies
King's College Hospital
Denmark Hill
London  SE5 9RS  UK
 
+44 (0)20 - 7346 - 3125 telefax
+44 (0)20 - 7346 - 4627 office