Re: Artefact on IHC slides

From:christine lee

I didn't see the original of this letter but I have seen students have a lot of artefact problems by using soft pencils to label slides, - graphite everywhere, all over the slides.
Christine Lee,
School of Molecular Biosciences,
University of Queensland
----- Original Message -----
From: Vnnie Della Speranza
To: ; ;
Sent: Thursday, October 31, 2002 8:59 AM
Subject: RE: Artefact on IHC slides

This is not an ihc artifact.
do you use sponges in your cassettes ? this looks to me that the biopsy came into contact with dry sponges which caused drying artifact in the tissue prior to immersion in fixative. the pattern of artifact is too regular to be consistent with Glen's suggestion of adhesion loss.
if I am correct, any lab that uses those blue or white sponges in their cassettes should saturate the sponges with formalin or whatever fixative you use to avoid drying of the sample when it comes into contact with the  needle bx.
do let us know if you discover the cause. great mystery!!
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> "Dawson, Glen" <> 10/30/02 03:05PM >>>

My guess is that it is an adhesion problem.  Maybe the slide that the tissue
was put on had a piece of gauze or something of the like sitting on it that
rubbed away the adhesive properties of the slide in a definite pattern.  In
the spots where the adhesion was gone, the tissue lifted trapping DAB under
the tissue.

Glen Dawson.

-----Original Message-----
Sent: Wednesday, October 30, 2002 1:26 PM
Subject: Artefact on IHC slides

Please view pictures on: and click on View List Serv Images
to view ipproblem1.jpg and ipproblem2.jpg.

The stain is IHC CD20 on a biopsy specimen using DAB as the chromogen and
Hematoxylin as the counterstain.

As far as I know, the tissue didn't come in contact with gauze and the
artifact pattern isn't consistent with our tissue cassettes.  Just curious
as to any/all answers on this one.

Laura Bliven
Marshfield Laboratories

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