This is not an ihc artifact.
do you use sponges in your cassettes ? this looks to me that the biopsy
came into contact with dry sponges which caused drying artifact in the tissue
prior to immersion in fixative. the pattern of artifact is too regular to be
consistent with Glen's suggestion of adhesion loss.
if I am correct, any lab that uses those blue or white sponges in their
cassettes should saturate the sponges with formalin or whatever fixative you
use to avoid drying of the sample when it comes into contact with the
needle bx.
do let us know if you discover the cause. great mystery!!
>>> "Dawson, Glen" <GDawson@Milw.Dynacare.com>
10/30/02 03:05PM >>>
Laura,
My guess is that it is an
adhesion problem. Maybe the slide that the tissue
was put on had a
piece of gauze or something of the like sitting on it that
rubbed away the
adhesive properties of the slide in a definite pattern. In
the spots
where the adhesion was gone, the tissue lifted trapping DAB under
the
tissue.
Glen Dawson.
-----Original Message-----
From:
bliven.laura@marshfieldclinic.org
[
mailto:bliven.laura@marshfieldclinic.org]Sent:
Wednesday, October 30, 2002 1:26 PM
To:
histonet@pathology.swmed.edu
Subject: Artefact on IHC
slides
Please view pictures on:
histonet.org and click on
View List Serv Images
to view ipproblem1.jpg and ipproblem2.jpg.
The
stain is IHC CD20 on a biopsy specimen using DAB as the chromogen
and
Hematoxylin as the counterstain.
As far as I know, the tissue
didn't come in contact with gauze and the
artifact pattern isn't consistent
with our tissue cassettes. Just curious
as to any/all answers on this
one.
Thanks,
Laura Bliven
Marshfield
Laboratories
bliven.laura@marshfieldclinic.org