Re: Artefact on IHC slides

From:Jan Shivers


I saw something similar to this also in recent weeks.  I noticed it in the
clear glass area of my slides, and it looked like the glass was slightly
etched in that waffle/houndstooth type pattern.  I attributed it to a bad
batch of glass slides.  It did look like the weave from a gauze sponge, but
it was unreasonable to assume that the gauze was pressed on EVERY single
glass slide the same way.  I'll be interested to see what other explanations
people come up with.

Jan Shivers

----- Original Message -----
Sent: Wednesday, October 30, 2002 1:26 PM
Subject: Artefact on IHC slides

> Please view pictures on:
> and click on View List Serv Images
> to view ipproblem1.jpg and ipproblem2.jpg.
> The stain is IHC CD20 on a biopsy specimen using DAB as the chromogen and
Hematoxylin as the counterstain.
> As far as I know, the tissue didn't come in contact with gauze and the
artifact pattern isn't consistent with our tissue cassettes.  Just curious
as to any/all answers on this one.
> Thanks,
> Laura Bliven
> Marshfield Laboratories

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