| From: | Tony Henwood |
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory
Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845
3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
-----Original Message-----Hello fellow Histonetters:
From: JPaulB42@aol.com [mailto:JPaulB42@aol.com]
Sent: Friday, 1 November 2002 4:25
To: histonet@pathology.swmed.edu
Subject: TRAcP
We have started experimenting with Zymed's TRAcP antibody with poor results on peripherial smears. Does anyone have any guidelines to follow to bring this antibody up and running? Washing off is a problem as well.
1. Fix in Acetone for 1 minute.
2. Steam retrieval in EDTA for 30 minutes.
3. Cool for 30 minutes.
4. Primary antibody for 60 minutes.
5. Link for 30 minutes (LSAB2)
6. Strep for 30 minutes.
7. DAB for 5 minutes
8. Counterstain with Hema for 5 - 8 seconds.
The procedure is run on DAKO Autostainer.
Thanks everyone for your help.
Jim Bradford
ORMC
Orlando, FL
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