RE: Schaffer fixation

From:"Nader, Alexander"

>I am looking for the recipe for this fixative.  I have:
>600 mL 80% ETOH
>300 mL 37% formol and CaCO3
>Does anyone know what the concentration of CaCO3 is?

we use 20 g of CaCO3 and 10g MgCO3 per litre and adjust the pH to 7,2 with
NaOH. Shake the solution once a week and store it at room temperature.

Dr. Alexander Nader
Path. Institut Hanuschkrankenhaus
Vienna, Austria

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