Stephen

We have been using MLH1 successfully, as well as the MSH2, MSH6, PMS2 and
MGMT antibodies (methodology accepted for publication in AIMM). We use the
Pharmingen MLH1 and carry out retrieval in a pressure cooker for 10 minutes
(using Citrate pH 6.0). Incubation is overnight at a dilution of 1/2000. If
you like I can send you our protocol.

Cheers

Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
email: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!


-----Original Message-----
From: Steve_Dalessandro@rsh.net [mailto:Steve_Dalessandro@rsh.net]
Sent: Saturday, 9 November 2002 11:31 PM
To: histonet@pathology.swmed.edu
Subject: MLH1 antibody



Still looking for some help with the MLH1 antibody. Is anyone doing the
stain at the moment and if yes .. how are you getting it to work??!

any help would be great

Stephen