I use this same antibody (clone G168-15) and it works fine. What detection
system are you using? Since you have a faint staining, you can try a more
sensitive detection system such as Vector Elite. You can also try Dako's
high pH retrieval solution. You should dilute further than recommended (over
1:50) to minimize the tissue damage and microwave about 20-40 min. For
routine fresh large bowel case, I use microwave AR and Envision detection
system. However, for the archival cases, I retrieve the slides in citrate
buffer pH6.0 with pressure cooker for 10-15 min. And I use Vecta Elite
system for staining. I also do overnight incubation (1:600 dil) for archival
cases. After boiling I do not leave the slide for slow cooling!
Any small or large bowel tissue can be a good positive control, and HNPCC
cases showing microsatellite instibility (showing the loss of MLH-1 or MSH-2
expression at the neoplastic epithelium) are not uncommon from our
I hope this helps.
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord NSW 2139 Australia
"the universe we observe has precisely the properties we should expect if
there is, at bottom, no design, no purpose, no evil and no good, nothing but
blind, pitiless indifference."
The information contained in this message is intended for the named
addressee only, and is confidential to the sender and intended recipient. If
you are not the named addressee please do not copy, distribute, take any
action reliant on, or disclose anything in this E-mail message to any other
person or organisation. If you have received this message in error please
delete the email and notify me immediately. Views expressed in this message
are those of the individual sender and are not necessarily the views of
Central Sydney Area Health Service.
> -----Original Message-----
> From: Klassen, Shannon SDH [mailto:email@example.com]
> Sent: Wednesday, 13 November 2002 02:32
> To: 'Histonet'
> Subject: MLH1
> Need help ASAP with MLH1 - BD Pharmingen #554072 - pretreatment
> /dilution/control? - Can't get it to work well - nuclear staining
> there but
> weak , and I can't take the dilution any lower. Thanks in advance.
> Shannon Klassen
> IHC Tech
> Regional Health Authority #6
> Saskatoon, Canada
> 306 655 8569
<< Previous Message | Next Message >>