From:Andrew Shand

You can make very nice preps by staining overnight in very dilute eosin.

Andy Shand

-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
Sent: 07 November 2002 13:04
To: Ian Montgomery;

We have had this subject a few times before. I am still at a loss to
understand the use of various "odd" stains for eosinophils.
They are so named because they like eosin. The easy way to stain them for
contrast (contrast being the idea of staining) is to do a H&E with very
light E. All the usual "E things" will be very lightly stained but with the
eosinophils as avidly stained as with a normal length of staining.
Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Consultant Histopathologist
Rotherham General Hospital, Yorkshire 

-----Original Message-----
From: Ian Montgomery []
Sent: 07 November 2002 10:15
Subject: Fwd: RE: Daily Digest

        Lendrum. 1944 J. Path. Bact. 54. 441.
        This method uses carbol chromotrope and works an absolute treat.

Date: Wed, 06 Nov 2002 16:07:10 -0800
From: "Aswani, Shakun {In-V~Palo Alto}" 
Subject: RE: Daily Digest
To: HistoNet Server 

I am looking for Staining protocol (which works) for Eosinophils. I have
tried several Congo Red methods but no luck. I am planning on doing Sirius
Red, I have ordered the dye hopefully that will work but in the mean time if
you have any suggestions please go for it I need all the help I can get.
call me direct 650-855-6432 or email me.
Thank you,

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