RE: Artefact on IHC slides

From:"Dawson, Glen"


My guess is that it is an adhesion problem.  Maybe the slide that the tissue
was put on had a piece of gauze or something of the like sitting on it that
rubbed away the adhesive properties of the slide in a definite pattern.  In
the spots where the adhesion was gone, the tissue lifted trapping DAB under
the tissue.

Glen Dawson.

-----Original Message-----
Sent: Wednesday, October 30, 2002 1:26 PM
Subject: Artefact on IHC slides

Please view pictures on: and click on View List Serv Images
to view ipproblem1.jpg and ipproblem2.jpg.

The stain is IHC CD20 on a biopsy specimen using DAB as the chromogen and
Hematoxylin as the counterstain.

As far as I know, the tissue didn't come in contact with gauze and the
artifact pattern isn't consistent with our tissue cassettes.  Just curious
as to any/all answers on this one.

Laura Bliven
Marshfield Laboratories

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