Paraffin infiltration by hand

From:Gayle Callis

Three changes MINIMUM at 4 hours per change, under vacuum, Tissue Prep 2
Fisher. My VIP had four changes of paraffin. 

At 10:31 AM 11/7/02 -0500, you wrote:
>Histonetters,
>
>It's me again from the ortho bone lab.  Besides asking about the best type
>of paraffin to use to process large bone sections, how long do you
>infiltrate a bone- size 5mm. in thickness that fits on a 5" x 7" slide?
>
>Thank you
>Peggy DiCarlo HT (ASCP)
>Buffalo General Hospital
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu




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