Mouse Immuno Question

From:Lynn Gruman

Hello histonetters,

For those of you doing immuno on mice here is our situation we have tissue 
that is both mouse and human (I know strange)... The antibody staining we 
want to repeat was great on human only tissue but unfortunately the 
antibodies we want to use not only recognize the human antigen but also 
recognize the mouse antigen as well. We only want the antigen to stain the 
human part of our experiment.  Is there any way to use these same 
antibodies (as we have tried others and they do not work well in paraffin) 
in a way they will only recognize the human antigen? In other words is 
there a way to block or purify the antibody so it will not recognize the 
mouse antigen.

Thanks everyone!

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