Mouse Immuno Question
Hello histonetters,
For those of you doing immuno on mice here is our situation we have tissue
that is both mouse and human (I know strange)... The antibody staining we
want to repeat was great on human only tissue but unfortunately the
antibodies we want to use not only recognize the human antigen but also
recognize the mouse antigen as well. We only want the antigen to stain the
human part of our experiment. Is there any way to use these same
antibodies (as we have tried others and they do not work well in paraffin)
in a way they will only recognize the human antigen? In other words is
there a way to block or purify the antibody so it will not recognize the
mouse antigen.
Thanks everyone!
Lynn
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