McFadyean's Methylene Blue
If you do a stain called McFadyean's Methylene Blue on paraffin sections or
if you do any stain specific for the capsule of Bacillus anthrasis, I would
like to hear from you. (No, we do not expect to see any......just want to
look some control blocks using a stain specific for the capsule.)
Through several internet searches I have found little about or references
about McFadyean's. I do know it is usually used on smears. It is a simple
stain using aged Loeffler's Methylene blue. That is can be rapidly oxidized
by the use of heat (Unna's polychrome). Thank you Dr. Kiernan. Your site of
FAQ was most helpful.
I am still searching through microbiologically staining sites. Any help
would be most appreciated. Any internet site suggestions appreciated.
But if I am really lucky, one of you will be my 'saving grace' and will be
able to share some valuable insight and/or experience with McFadyean's
Barbara H. Stancel, HTL(ASCP)HT
USDA, FSIS, OPHS, Eastern Laboratory, Pathology
RRC, 950 College Station Road
Athens, Georgia 30604
phone: (706) 546-3556
fax: (706) 546-3589
Unlimited Internet access -- and 2 months free!#160# Try MSN.
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