McFadyean's Methylene Blue

From:Barbara Stancel

If you do a stain called McFadyean's Methylene Blue on paraffin sections or 
if you do any stain specific for the capsule of Bacillus anthrasis, I would 
like to hear from you. (No, we do not expect to see any......just want to 
look some control blocks using a stain specific for the capsule.)

Through several internet searches I have found little about or references 
about McFadyean's. I do know it is usually used on smears. It is a simple 
stain using aged Loeffler's Methylene blue. That is can be rapidly oxidized 
by the use of heat (Unna's polychrome). Thank you Dr. Kiernan. Your site of 
FAQ was most helpful.

I am still searching through microbiologically staining sites. Any help 
would be most appreciated.  Any internet site suggestions appreciated.

But if I am really lucky, one of you will be my 'saving grace' and will be 
able to share some valuable insight and/or experience with McFadyean's 
Methylene Blue.

Histologically yours,

Barbara H. Stancel, HTL(ASCP)HT
USDA, FSIS, OPHS, Eastern Laboratory, Pathology
RRC, 950 College Station Road
Athens, Georgia  30604
phone: (706) 546-3556
fax: (706) 546-3589

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