BD Zinc formalin free fix and perfusion

From:Jan Bauer

>Dear Histonetters,

I have two questions about the Zinc formalin free fixative. I HAVE gone 
through the histonet archives but cannot find an answer to these questions:

1) Has someone ever tried perfusion of mice/rat with this fixative: from 
John Kiernanīs messages we know that we cannot use Phophate buffered 
buffers before using the Zinc fixative, but it should be possible to=20
perfuse first with saline or?

2) If the Zinc fixative works well and gives a not too bad morphology after 
paraffin embedding, why are then so few people using it?

Any answers or thought about these questions are much appreciated (Gayle, 


Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna

tel:    +43-1-4277 62813
fax:    +43-1-4277 9628

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