Artefact on IHC slides

From:bliven.laura@marshfieldclinic.org


Please view pictures on:
histonet.org and click on View List Serv Images
to view ipproblem1.jpg and ipproblem2.jpg.

The stain is IHC CD20 on a biopsy specimen using DAB as the chromogen and Hematoxylin as the counterstain.

As far as I know, the tissue didn't come in contact with gauze and the artifact pattern isn't consistent with our tissue cassettes.  Just curious as to any/all answers on this one.

Thanks,
Laura Bliven
Marshfield Laboratories
bliven.laura@marshfieldclinic.org




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