markers for arteries or veins
We are interested in differentiating between arteries and veins in the adult rat pulmonary vasculature. Are there specific markers or antibodies we could use? A variety of plant lectins exists which label endothelial cells but the literature is confusing about their specificities. They may also preferentially stain only certain organs.
We are perfusing the rats, and cutting thick sections (~100 microns) on a vibratome for confocal microscopy. So staining could be carried out either at the time of perfusion or later, with the sections. A fluorescent marker is required for the confocal technique.
I look forward to any suggestions.
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8
ph: 416-864-6060 x6337
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