cutting whole mice
i have been asked about cutting whole young mice treated with beta-gal.
i did this at one time as frozen sections by placing a microtome in a
chest freezer and using a tungsten carbide knife. question: in order
to sucessfully do ihc for beta-gal would the sample have to be frozen or
does it stand up to processing?
the exact inquiry follows.
I was given your name by Nancy Shellhorn and she thought you would be
just the person to help me!
I work in a core lab which provides histology support to many users. I
have an investigator who is interested in doing whole mounts of young
mice. He is injecting them with Beta-Gal at birth, then wants to look
for the stain after they have grown a little- he says they will be
"young mice" but hasn't given me an exact age. I believe weeks as
opposed to months.
Have you any experience processing entire small mice and cutting them?
If not, can you advise me of a source (human or literature) that may be
able to assist me?
Thank You Very Much,
<< Previous Message | Next Message >>