Re: markers for arteries or veins
You probably already know that ephrin B2 is expressed in arteries
and Eph-B4 in veins (Cell 1998 May 29;93(5):741-53).
I don't know if there are any antibodies available. But
you could use in situ hybridization :-)
Mikael Niku URL: www.helsinki.fi/~mniku/
University of Helsinki Dept. Basic Veterinary Sciences
- Mitäkö mieltä olen länsimaisesta sivistyksestä?
Minusta se olisi erinomainen ajatus!
----- Original Message -----
From: "Judy Trogadis"
Sent: Wednesday, November 28, 2001 5:02 PM
Subject: markers for arteries or veins
> We are interested in differentiating between arteries and veins in the
adult rat pulmonary vasculature. Are there specific markers or antibodies we
could use? A variety of plant lectins exists which label endothelial cells
but the literature is confusing about their specificities. They may also
preferentially stain only certain organs.
> We are perfusing the rats, and cutting thick sections (~100 microns) on a
vibratome for confocal microscopy. So staining could be carried out either
at the time of perfusion or later, with the sections. A fluorescent marker
is required for the confocal technique.
> I look forward to any suggestions.
> Thank you
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 8Q
> 30 Bond St.
> Toronto, ON M5B 1W8
> ph: 416-864-6060 x6337
> fax: 416-864-6043
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