Re: markers for arteries or veins

From:Mikael Niku

Dear Judy,

You probably already know that ephrin B2 is expressed in arteries
and Eph-B4 in veins (Cell 1998 May 29;93(5):741-53).

I don't know if there are any antibodies available. But
you could use in situ hybridization :-)

   Mikael Niku             URL:
   University of Helsinki  Dept. Basic Veterinary Sciences
       - Mitäkö mieltä olen länsimaisesta sivistyksestä?
         Minusta se olisi erinomainen ajatus!
                                              - Gandhi

----- Original Message -----
From: "Judy Trogadis" 
Sent: Wednesday, November 28, 2001 5:02 PM
Subject: markers for arteries or veins

> We are interested in differentiating between arteries and veins in the
adult rat pulmonary vasculature. Are there specific markers or antibodies we
could use? A variety of plant lectins exists which label endothelial cells
but the literature is confusing about their specificities. They may also
preferentially stain only certain organs.
> We are perfusing the rats, and cutting thick sections (~100 microns) on a
vibratome for confocal microscopy. So staining could be carried out either
at the time of perfusion or later, with the sections. A fluorescent marker
is required for the confocal technique.
> I look forward to any suggestions.
> Thank you
> Judy
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 8Q
> 30 Bond St.
> Toronto, ON M5B 1W8
> Canada
> ph:  416-864-6060  x6337
> fax: 416-864-6043

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