Re: help need to make Carnoy's fluid
On Sun, 25 Nov 2001, Dr Anton Perera wrote from Sri Lanka:
> I am using Carnoy's fluid for cancer treatments
Carnoy's fluid is a fixative, not a drug.
> To be effective I like to make it by myself
> So I would more appreciate if u can tell me how to
> make it. What are the ingredients?
> I need a good description
It is very easy.
Ethanol 60 ml
Chloroform 30 ml
Acetic acid 10 ml
Fix by immersion, 4 to 18 hours. Short time for
small specimens, overnight for bigger ones.
Transfer to either 100% alcohol to proceed with
processing into paraffin, or 70% alcohol if
specimens must be held for a few days before
processing and embedding.
I'm sending this reply also to the Histonet listserver,
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John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
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