Re: help need to make Carnoy's fluid

From:"J. A. Kiernan"

On Sun, 25 Nov 2001, Dr Anton Perera wrote from Sri Lanka:

> I am using Carnoy's fluid for cancer treatments

Carnoy's fluid is a fixative, not a drug.

> To be effective I like to make it by myself
> So I would more appreciate if u can tell me how to
> make it. What are the ingredients?
> I need a good description

It is very easy.

  Ethanol      60 ml
  Chloroform   30 ml
  Acetic acid  10 ml

Fix by immersion, 4 to 18 hours. Short time for
small specimens, overnight for bigger ones.

Transfer to either 100% alcohol to proceed with
processing into paraffin, or 70% alcohol if
specimens must be held for a few days before
processing and embedding. 

I'm sending this reply also to the Histonet listserver,
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----------------------------------------
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan
 





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