Re: help need to make Carnoy's fluid

From:"J. A. Kiernan"

On Sun, 25 Nov 2001, Dr Anton Perera wrote from Sri Lanka:

> I am using Carnoy's fluid for cancer treatments

Carnoy's fluid is a fixative, not a drug.

> To be effective I like to make it by myself
> So I would more appreciate if u can tell me how to
> make it. What are the ingredients?
> I need a good description

It is very easy.

  Ethanol      60 ml
  Chloroform   30 ml
  Acetic acid  10 ml

Fix by immersion, 4 to 18 hours. Short time for
small specimens, overnight for bigger ones.

Transfer to either 100% alcohol to proceed with
processing into paraffin, or 70% alcohol if
specimens must be held for a few days before
processing and embedding. 

I'm sending this reply also to the Histonet listserver,
which is the internet forum for enquiries such as
yours. If you have other questions about histotechnology
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John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1

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