Re: fibrin stain

From:Lee & Peggy Wenk

Also known at the MSB stain, short for
Martius yellow
brilliant crystal Scarlet
Soluble Blue (aka methyl blue)

It's essentially a standard Masson trichrome, but
Martius yellow has been added to stain the red blood
cells yellow, so that the red fibrin can be seen easier.
With the regular Masson trichrome, the red fibrin
can be difficult to see if there are a lot of RBC. The
MSB makes it easier to see the fibrin amongst
the RBC.

If you don't want to do the MSB, do the
Masson Trichrome or the PTAH. Both
will demonstrate fibrin.

I had problems finding the brilliant crystal scarlet,
so substituted our regular red dye from the Masson
trichrome, and it worked fine. (Any red medium
dye used for Masson trichrome would work)

I also substituted aniline blue for soluble blue, since that
is what we had at work, and it worked too. (Any large
blue or green dye used for Masson or Gomori trichrome
would work.)

It also calls for a double nuclear staining, celestine blue
and hematoxylin. Since we don't do this stain very often,
it was an inconvenience to make up celestine blue. So I
substituted Weigert hematoxylin for both hematoxylins,
and it worked fine. In  fact, our pathologists like it better
with the black nuclei.

I'll send you our procedure from work.

It is listed in the 3rd edition of the Bancroft
and Stevens book "Theory and Practice of
Histological Techniques." I don't know about
their later editions. I'm at home, and this is
the latest edition I have here.

If anyone else would like the procedure, email me at
work, and I'll send it as an attachment in Word format.

Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Jill Songer" 
Sent: Thursday, November 15, 2001 12:35 PM
Subject: fibrin stain

> One of our pathologists has asked for the Frazier-Lendrum stain for
> Would some kind soul please share their procedure?
> My fax is below. Thanks so much.
> **************************************************************************
> Jill Songer HT (ASCP)
> Supervisor, Anatomic Pathology
> Veterinary Medical Teaching Hospital
> Virginia Tech
> Fax-540-231-2657

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