Re: cutting whole mice

From:Jamie Erickson

        We've only do B-galactosidase staining on frozens. I don't know if this is what your asking but we treat mice with adenovirus that express the marker  gene B-galactosidase then freeze the tissues  (after days of injection of the virus ) for staining and using the enzymatic method (X-gal, Ferric and Ferro cyanide etc..) and detect B-gal in various tissues. I have not used the B-gal antibodies for B-gal staining, the enzymatic method is a lot easier. Hope this helps..

Jamie Erickson
Scientist II
Wyeth/Genetics Institute
1 Burtt Rd.
Andover, MA   01810
work : (978) 247-1348
FAX  : (978) 247-1389

>>> rueggp  11/26 5:35 PM >>>
fellow netters,
i have been asked about cutting whole young mice treated with beta-gal.
i did this at one time as frozen sections by placing a microtome in a
chest freezer and using a tungsten carbide knife.  question:  in order
to sucessfully do ihc for beta-gal would the sample have to be frozen or
does it stand up to processing?
the exact inquiry follows.
Dear Patsy,
I was given your name by Nancy Shellhorn and she thought you would be
just the person to help me!
I work in a core lab which provides histology support to many users.  I
have an investigator who is interested in doing whole mounts of young
mice.  He is injecting them with Beta-Gal at birth, then wants to look
for the stain after they have grown a little- he says they will be
"young mice" but hasn't given me an exact age.  I believe weeks as
opposed to months.
Have you any experience processing entire small mice and cutting them?
If not, can you advise me of a source (human or literature) that may be
able to assist me?

Thank You Very Much,
Marda Jorgensen

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