Re: West Nile Virus Antibody

From:Louri Caldwell

Hi Cheryl,

The Southeastern Cooperative Wildlife Disease Service, SCWDS, out of this 
University has been doing IHC for West Nile Virus for some time. Over the 
past several months, since the virus was found here in the Southeast, they 
have been inundated with samples - performing one to two runs every day to 
keep up with the load.

They are using a polyclonal antibody purchased commercially from 
BioRealiance in Rockville, Maryland (phone 301-738-1000).  The technician I 
spoke with has been very pleased with the performance of this antibody but 
noted it will cross with, she believed, St. Louis Encephalitis.  While the 
retrieval required may be different for your lab, they use Ventanna's 
protease for 2-3 minutes before staining.
BioRealiance also offers a monoclonal antibody, which SCWDS has purchased 
but as yet has not tested.  The tech also noted both antibodies were 
inexpensive - always a good thing!

If you need any more information, please feel free to contact me. Hope this 


Louri Caldwell, BS, HTL (ASCP)
Chief Histotechnologist
College of Veterinary Medicine
University of Georgia
Athens, Georgia 30602
(706) 542-5835

>From: Cheryl Crowder 
>To: histonet 
>Subject: West Nile Virus Antibody
>Date: Mon, 26 Nov 2001 09:59:54 -0600
>Good morning - Do any of you out there know where we can obtain antibody
>to West Nile virus?  If you know of a source would you please contact
>me?  Thank you,  Cheryl
>Cheryl Crowder, BA, HTL(ASCP)
>Chief Technologist
>Department of Pathobiological Sciences
>School of Veterinary Medicine
>Louisiana State University
>Baton Rouge, LA  70803
>(225) 578-9734
>FAX  (225) 578-9720

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