Re: Unstained Slide IHC Preservation
I would be careful about simply dipping in paraffin. When slides are stored, the
paraffin is not sealed on the section and can either form a pocket of air or can
often separate and leave the section itself exposed. I prefer to make sure that
the wax melts with the wax of the section and then is quickly solidified.
Rose Richardson wrote:
> Great idea about dipping in paraffin, if it works for the block it sure
> would for the slide.
> ----- Original Message -----
> From: "Jenny Oblander"
> To: "'Dawson, Glen'" ; "HistoNet Server"
> Sent: Wednesday, November 21, 2001 11:36 AM
> Subject: RE: Unstained Slide IHC Preservation
> > I have used two methods in the past to retain slides. one is to freeze
> > (only if you have unlimited space in your -20), or dip the slide in
> > and store in a slide box, I have stored slides(coated with paraffin) for
> > seven years and still have gotten excellent results from IHC. Jenny
> > -----Original Message-----
> > From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
> > Sent: Wednesday, November 21, 2001 8:39 AM
> > To: HistoNet Server; Dawson, Glen
> > Subject: RE: Unstained Slide IHC Preservation
> > Histonetters,
> > I have a pathologist who wishes to preserve some unstained slides for
> > Immunohistochemical analysis. The blocks must be returned so all he has
> > work with is unstained. He estimates that it may be upwards of 10 years
> > before the IHC would be done on these slides and would like to know the
> > way to preserve the desired antigens on the slides. I have done IHC on 10
> > year old blocks but never on such old unstained slides. Any suggestion on
> > the best way to preserve these slides would be greatly appreciated.
> > Thank-you in Advance,
> > Glen A. Dawson BS, HT & IHC (ASCP)
> > Lead IHC Technologist
> > Milwaukee, WI
<< Previous Message | Next Message >>