Re: TTF-1
Shanon,
Our results
were inconsistent at first. We tried cutting only a small supply of
slides and storing them in the refrigerator. Saw an immediate difference.
but still noticed a gradual decrease in staining over a couple of weeks.
Made the ab fresh used the slides stored in the fridge the intensity was
back. So now we store these controls in the fridge,(older slides stored
at room temp stained lighter) and make up only 1ml of TTF-1 at a
time. By doing both our results are consistenly good!
I know
that several people on the histonet have suggested freezer storage, but
our freezer is nearly full so this waas not an option.
Rena Fail, AS, HT(ASCP)
IHC/SS
Medical University of SC
charleston, SC
At 01:29 PM 11/27/01 -0600, you wrote:
I'M TRYING TO GET MY TTF-1 ANTIBODY
FROM DAKO WORKING AND AM GETTING SOME
INCONSISTENT RESULTS. I WAS WONDERING IF SOMEONE COULD SUPPLY ME WITH A
FEW
CONTROL SLIDES TO SEE IF I'M IN THE RIGHT BALLPARK OR WAY OFF BASE - I
CAN'T
SEEM TO PEG DOWN A DECENT CONTROL EITHER.
SHANNON KLASSEN
IHC TECH
SASKATOON DISTRICT HEALTH
SASKATOON, CANADA
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