Gayle, 

We have a new product called Cal-Arrest that will quickly stop
decalcification but will not dehydrate tissue as 70% alcohol will.
Pharmaceutical and Veterinary Researchers seem to love it !!


-- 
Cliff Berger
1-800-428-5856
You probably use or name, why not use our product?
www.decal-bone.com
Decal is a registered trademark of Decal Chemical Corp.

> From: rueggp 
> Date: Wed, 28 Nov 2001 14:34:54 -0700
> To: Gayle Callis 
> Cc: Lee & Peggy Wenk , histonet@pathology.swmed.edu
> Subject: Re: Post-fixation of bone marrow specimens
> 
> Gayle,
> i wondered about that also and i thought maybe they were trying to remove the
> percipitate????
> Patsy
> 
> Gayle Callis wrote:
> 
>> Peggy,
>> 
>> Just a curious question about why you rinse away B-5 with 70% alcohol
>> before decalcification rather than a gentle short rinse with running tap
>> water?
>> 
>> 70% alcohol is frequently used to stop acid decalcification and will
>> actually slow down, even prevent decalcification until the tissue/bone
>> marrow rehydrates in the aqueous acid mixture.  We used 70% alcohol for
>> years to hold partially decalcified (near endpoint) bone samples overnight
>> in order to not overdecalcify then opted to not to do it due to described
>> reasons.
>> 
>> 
>> 
>> At 08:26 PM 11/27/01 -0500, you wrote:
>>> If the tissue is fixed in B5, why does it need to be placed in NBF
>>> as a second fixative? Just go from B5, rinse in 70% alcohol for
>>> at least 30 minutes, then go into decal. That's what we do.
>>> 
>>> Peggy A. Wenk, HTL(ASCP)SLS
>>> William Beaumont Hospital
>>> Royal Oak, MI 48073
>>> 
>>> ----- Original Message -----
>>> From: "Richard Cartun" 
>>> To: 
>>> Sent: Tuesday, November 27, 2001 2:07 PM
>>> Subject: Post-fixation of bone marrow specimens
>>> 
>>> 
>>>> Is there a minimum time for a B5-fixed bone marrow core biopsy to be
>>> post-fixed in 10% buffered formalin before decalcification?  Thanks!
>>>> 
>>>> R. Cartun
>>>> 
>>>> 
>>>> 
>>> 
>>> 
>>> 
>>> 
>> Gayle Callis
>> MT,HT,HTL(ASCP)
>> Histopathology Supervisor
>> Veterinary Molecular Biology - Marsh Lab
>> Montana State University - Bozeman
>> 19th and Lincoln St
>> Bozeman MT 59717-3610
>> 
>> 406 994-6367
>> 406 994-4303 (FAX)
> 
>