Re: Post-fixation of bone marrow specimens
We have a new product called Cal-Arrest that will quickly stop
decalcification but will not dehydrate tissue as 70% alcohol will.
Pharmaceutical and Veterinary Researchers seem to love it !!
You probably use or name, why not use our product?
Decal is a registered trademark of Decal Chemical Corp.
> From: rueggp
> Date: Wed, 28 Nov 2001 14:34:54 -0700
> To: Gayle Callis
> Cc: Lee & Peggy Wenk , firstname.lastname@example.org
> Subject: Re: Post-fixation of bone marrow specimens
> i wondered about that also and i thought maybe they were trying to remove the
> Gayle Callis wrote:
>> Just a curious question about why you rinse away B-5 with 70% alcohol
>> before decalcification rather than a gentle short rinse with running tap
>> 70% alcohol is frequently used to stop acid decalcification and will
>> actually slow down, even prevent decalcification until the tissue/bone
>> marrow rehydrates in the aqueous acid mixture. We used 70% alcohol for
>> years to hold partially decalcified (near endpoint) bone samples overnight
>> in order to not overdecalcify then opted to not to do it due to described
>> At 08:26 PM 11/27/01 -0500, you wrote:
>>> If the tissue is fixed in B5, why does it need to be placed in NBF
>>> as a second fixative? Just go from B5, rinse in 70% alcohol for
>>> at least 30 minutes, then go into decal. That's what we do.
>>> Peggy A. Wenk, HTL(ASCP)SLS
>>> William Beaumont Hospital
>>> Royal Oak, MI 48073
>>> ----- Original Message -----
>>> From: "Richard Cartun"
>>> Sent: Tuesday, November 27, 2001 2:07 PM
>>> Subject: Post-fixation of bone marrow specimens
>>>> Is there a minimum time for a B5-fixed bone marrow core biopsy to be
>>> post-fixed in 10% buffered formalin before decalcification? Thanks!
>>>> R. Cartun
>> Gayle Callis
>> Histopathology Supervisor
>> Veterinary Molecular Biology - Marsh Lab
>> Montana State University - Bozeman
>> 19th and Lincoln St
>> Bozeman MT 59717-3610
>> 406 994-6367
>> 406 994-4303 (FAX)
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