Re: Post-fixation of bone marrow specimens
I agree with Peggy, we didn't even wash with alcohol, I assume that is to
remove the mercury percipitate.
Lee & Peggy Wenk wrote:
> If the tissue is fixed in B5, why does it need to be placed in NBF
> as a second fixative? Just go from B5, rinse in 70% alcohol for
> at least 30 minutes, then go into decal. That's what we do.
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
> ----- Original Message -----
> From: "Richard Cartun"
> Sent: Tuesday, November 27, 2001 2:07 PM
> Subject: Post-fixation of bone marrow specimens
> > Is there a minimum time for a B5-fixed bone marrow core biopsy to be
> post-fixed in 10% buffered formalin before decalcification? Thanks!
> > R. Cartun
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