Gayle,
i wondered about that also and i thought maybe they were trying to remove the
percipitate????
Patsy

Gayle Callis wrote:

> Peggy,
>
> Just a curious question about why you rinse away B-5 with 70% alcohol
> before decalcification rather than a gentle short rinse with running tap
> water?
>
> 70% alcohol is frequently used to stop acid decalcification and will
> actually slow down, even prevent decalcification until the tissue/bone
> marrow rehydrates in the aqueous acid mixture.  We used 70% alcohol for
> years to hold partially decalcified (near endpoint) bone samples overnight
> in order to not overdecalcify then opted to not to do it due to described
> reasons.
>
>
>
> At 08:26 PM 11/27/01 -0500, you wrote:
> >If the tissue is fixed in B5, why does it need to be placed in NBF
> >as a second fixative? Just go from B5, rinse in 70% alcohol for
> >at least 30 minutes, then go into decal. That's what we do.
> >
> >Peggy A. Wenk, HTL(ASCP)SLS
> >William Beaumont Hospital
> >Royal Oak, MI 48073
> >
> >----- Original Message -----
> >From: "Richard Cartun" 
> >To: 
> >Sent: Tuesday, November 27, 2001 2:07 PM
> >Subject: Post-fixation of bone marrow specimens
> >
> >
> >> Is there a minimum time for a B5-fixed bone marrow core biopsy to be
> >post-fixed in 10% buffered formalin before decalcification?  Thanks!
> >>
> >> R. Cartun
> >>
> >>
> >>
> >
> >
> >
> >
> Gayle Callis
> MT,HT,HTL(ASCP)
> Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
> 406 994-6367
> 406 994-4303 (FAX)