Re: Post-fixation of bone marrow specimens

From:Gayle Callis


Just a curious question about why you rinse away B-5 with 70% alcohol
before decalcification rather than a gentle short rinse with running tap

70% alcohol is frequently used to stop acid decalcification and will
actually slow down, even prevent decalcification until the tissue/bone
marrow rehydrates in the aqueous acid mixture.  We used 70% alcohol for
years to hold partially decalcified (near endpoint) bone samples overnight
in order to not overdecalcify then opted to not to do it due to described


At 08:26 PM 11/27/01 -0500, you wrote:
>If the tissue is fixed in B5, why does it need to be placed in NBF
>as a second fixative? Just go from B5, rinse in 70% alcohol for
>at least 30 minutes, then go into decal. That's what we do.
>Peggy A. Wenk, HTL(ASCP)SLS
>William Beaumont Hospital
>Royal Oak, MI 48073
>----- Original Message -----
>From: "Richard Cartun" 
>Sent: Tuesday, November 27, 2001 2:07 PM
>Subject: Post-fixation of bone marrow specimens
>> Is there a minimum time for a B5-fixed bone marrow core biopsy to be
>post-fixed in 10% buffered formalin before decalcification?  Thanks!
>> R. Cartun
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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