If the tissue is fixed in B5, why does it need to be placed in NBF
as a second fixative? Just go from B5, rinse in 70% alcohol for
at least 30 minutes, then go into decal. That's what we do.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Richard Cartun" 
To: 
Sent: Tuesday, November 27, 2001 2:07 PM
Subject: Post-fixation of bone marrow specimens


> Is there a minimum time for a B5-fixed bone marrow core biopsy to be
post-fixed in 10% buffered formalin before decalcification?  Thanks!
>
> R. Cartun
>
>
>