RT-PCR on Paraffin sections

From:James Mubiru

Hullo everybody

I have a question on using formalin fixed paraffin embeded tissue sections for In Situ RT-PCR studies. I have been told that almost all of the RNA is degrded by the tissue processing procedures that it is almost a waste of time. I have been told to instead try frozen sections. But I love the cell detail I see with paraffin sections such that I want to give paraffin sections a try. My question to you out there is that has anybody tried to do In Situ RT-PCR on paraffin sections and what their experience was. I have heard of a product called RNAlater from Ambion. Has anybody used it for paraffin sections and what was their experience with it.

James Mubiru

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