RT-PCR FFPE Methodology

From:Jeff Silverman

Dr Hiroshi Hashimoto in Kitakyushu Japan and his group study all kinds of
soft tissue tumors for fusion transcript proteins resukltibng from genetic
mutations. Any of his group's papers will have full materials and methods
for successful RT-PCR on FFPE tissue.

J Pathol 2001 May;194(1):88-94
Expression of COL1A1-PDGFB fusion transcripts in superficial adult
fibrosarcoma suggests a close relationship to dermatofibrosarcoma

Sheng WQ, Hashimoto H, Okamoto S, Ishida T, Meis-Kindblom JM, Kindblom LG,
Hisaoka M.

Hiroshi Hashimoto, MD. Department of Pathology and Oncology, School of
Medicine, University of Occupational and Environmental Health (UOEH), 1-1
Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.

Hope this helped.
Jeff Silverman
Southside Hospital
Bay Shore NY USA

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