RE: Unstained Slide IHC Preservation

From:Cynthia Favara

If it were up to me I would cut sections, put them in a plastic slides box
with desiccators and then freeze @ -80. I have cut extra slides and gone
back 5-7 years later with some antibodies and been successful but with some
antibodies I notice a loss even with 2-3 weeks sitting on the bench. No
controlled study just my observation.

Cynthia Favara
Rocky Mountain Laboratories
903 S. 4th Street
Hamilton, MT 59840
FAX 406-363-9286

-----Original Message-----
From: Dawson, Glen []
Sent: Wednesday, November 21, 2001 7:39 AM
To: HistoNet Server; Dawson, Glen
Subject: RE: Unstained Slide IHC Preservation


I have a pathologist who wishes to preserve some unstained slides for future
Immunohistochemical analysis.  The blocks must be returned so all he has to
work with is unstained.  He estimates that it may be upwards of 10 years
before the IHC would be done on these slides and would like to know the best
way to preserve the desired antigens on the slides.  I have done IHC on 10
year old blocks but never on such old unstained slides.  Any suggestion on
the best way to preserve these slides would be greatly appreciated.

Thank-you in Advance,

Glen A. Dawson  BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI 

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