I put 0.002% NaAzide in things keep @4C for years with no problem. Other
option which I use for diluents is to make fresh weekly sterile filter and
keep@4C. You can freeze aliquots of what ever protein source you use[BSA,
Fetal calf serum, normal goat normal rabbit etc]and make quickly.

Cynthia Favara

-----Original Message-----
From: Klassen, Shannon SDH [mailto:klassens@sdh.sk.ca]
Sent: Tuesday, November 27, 2001 12:45 PM
To: 'Histonet'
Subject: PRIMARY DILUENT


I MAKE MY OWN PRIMARY DILUENT FOR DILUTING MY PRIMARY ANTIBODIES - 1% BSA -
WE USUALLY GO THOUGHT  IT SO FAST THAT I DON'T HAVE TO USE A ANTIBACTERIAL
AGENT IN IT BUT I AM GETTING "BUGS" AND I AM GUESSING IT IS PROBABLY THE
DILUENT SO DOES ANYONE HAVE A RECIPE FOR ME FOR 1%BOVINE SERUM ALBUMIN WITH
SODIUM AZIDE IN IT? 
SHANNON KLASSEN
IHC TECH
SASKATOON DISTRICT HEALTH
SASKATOON, CANADA