RE: Alcian blue for marking margins

From:Bruce Gapinski

Yep. Bruce

		-----Original Message-----
		From:	Weems, Joyce []
		Sent:	Friday, November 30, 2001 5:25 AM
		To:	'Bruce Gapinski'
		Subject:	RE: Alcian blue for marking margins

		Get this at the grocery?
		Joyce Weems
		Pathology Manager
		Saint Joseph's Hospital of Atlanta

			-----Original Message-----
			From:	Bruce Gapinski
			Sent:	Thursday, November 29, 2001 2:44 PM
			To:	'Aidan Schurr'
			Cc:	''
			Subject:	RE: Alcian blue for marking margins

			Save time & $. Use Mrs. Stewerts liquid bluing. I've
been using it
			years. Bruce Gapinski HT(ASCP)

					-----Original Message-----
					From:	Aidan Schurr
					Sent:	Thursday, November 29, 2001
11:16 AM
					Subject:	Re: Alcian blue for
marking margins


					Went through this same dilemma about
a year ago.
		Have you
			tried different "indian" inks?  I quickly discovered
that all so
			indian inks are definitely not created equal...  I
had to trial
		about a
			dozen or so before finding a suitable one.  The ink
currently used
			(unfortunately for you, fortunately for us)
manufactured here in New
			Zealand, and goes onto wet or dry, fixed or fresh
tissue with little
			Once on it stays there.  Really good microscopic
marking - obvious
		layer, no
			'weak' spots, no 'bacteria-like' granules at high
power.  Best of
		all it
			costs about $30NZ for a litre (that's about 10
pounds, or $15US [per
			for you guys!!]).  We also have a couple of other
inks designed for
			technical drawing pens (as an architect would use)
made by "Rotring"
			be available virtually anywhere).  We use their Blue
and Green,
		which we
			find have similar characteristics to the black, but
are more

					Best of luck


					aidan schurr
					section head, histology
					hutt valley district health board
					lower hutt
					new zealand
					++64 4 570 9173

					>>> RSH Histology

		30/11/2001 >>>
					With difficulties in obtaining
adequate quality (and
			affordable) Alcian blue
					8GX in the UK, a problem I suspect
		elsewhere, I
			would be
					interested to hear of alternative
dyes/methods in
			Indian ink has not
					proved to be
					"consultant friendly" and commercial
"margin kits"
		have not
					processing and staining "fast" here.
Any suggestions
			useful alternative
					dyes etc gratefully
					Good quality Alcian blue 8GX is our
gold standard
		(clean in
			use, easy to
					paint/dip, perfect for microscopy
with H&E) but it's
			disappearing fast!

					Jim Almond
					Head BMS
					Royal Shrewsbury Hospital


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