Mouse lens/cataracts - membranes!!

From:Bruce Abaloz

Dear All, my name is Monica & I'm working on a mouse knockout model(genetically modified) that develops cataracts at about 35 days of age. I'm particularly interested in looking at any alterations in protein or water distribution in the cataractous lens, also at the membranes of the lens cells/fiber cells and if possible the extracellular space. I have tried using Mallory's triple stain which has showed up some remarkable differences between pre and post cataract lenses, but I don't have an explanation for what each colour means as it's conventionally used for collagen staining. Any ideas on the best stain to use to look at these features or an explanation of the Mallory stain in this application would be appreciated....Cheers,

Monica (Melbourne Uni. OZ)
    **********************************************************************            BRUCE ABALOZ                           PH:61383446282
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