Helloo Histo techs....
I am having a very difficult time getting good tissues of  Finch brains. I 
fix tissue in 4% paraformaldehyde. Afterwards in series of sucrose b4 gelatin 
embedding then fast freeze.  After cutting, (-12 c in cryostat)I see "swiss 
cheese" type of tissue. It's wht our lab call tissue lysing. I can't figure 
out wht's going on....... can anyone help me? pls?
thanks
G.F.