[Fwd: Re: Fixation of entire lung]


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Phillip Huff
FBN Dummerstorf

-------- Original Message --------
Subject: Re: Fixation of entire lung
Date: Thu, 22 Nov 2001 12:00:43 +0100
From: huff <huff@fbn-dummerstorf.de>
To: jjthorsten@mmm.com
CC: histonet@pathology.swmed.edu
References: <OF616868E6.EC73B575-ON86256B0B.005A5D3C@mmm.com>

The only technique that I can recommend is something I saw on exhibit in 
Berlin over the summer called Plastination (see 
http://www.plastination.com). The technique can be applied at the whole 
tissue, organ, or even body level. The technique outlined on the webpage 
is for organ slices but the process is also applied to whole organs. The 
samples that I saw were quite pliable and completely intact. The process 
includes fixation, dehydration, forced impregnation of a polymer, and 
finally curing. I would recommend that you contact them for your 
particular needs. Contact information is on their web page. The result 
is amazing.
I don't know though if the final tissue can be used for histochemical 
processing. You will have to ask them. If you decide to use their 
process, please post a follow-up. I would be interested to see what they 
can offer. You can view some of the resulting "whole human body" 
preparations on their exhibition website 
(http://www.koerperwelten.com/index_en.htm). One of the most popular 
exhibits was actually two lungs, one was from a smoker, and the other 
from a non-smoker. The former was quite healthy looking and the latter 
looked like a poorly paved highway. If you are interested in the image 
to see the level of detail, I have a copy I could send you via email.

Hope this help,
Phillip Huff
FBN Dummerstorf

jjthorsten@mmm.com wrote:

> Hi histonetters,
> I am wondering if anyone knows a way to fix an entire lung to keep it as
> pliable as possible, but also to keep it from decomposing.  I have seen in
> some educational catalogs that they have model lungs which are from pigs
> for inhalation demonstrations.  Any information you can provide would be
> great.
> Thank You,
> Jason
> HT, (ASCP)

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