Hi Histonetters

Is there a scientific explanation for the use of calcium chloride in
trypsin when digesting tissue prior to IHC staining. I use trypsin on its
own, but for certain antibodies, the addition of calcium chloride to the
trypsin solution tends to aid in the digestion of tissue. Any comments?

thanks

Zenobia Haffajee  


Zenobia Haffajee
Anatomical Pathology 
HAPS John Hunter Hospital
dpeppera@mail.newcastle.edu.au
Tel :(02) 4921 4449
Fax :(02) 4921 4507