fixation of mucinous fluid in small airways?

From:LaCinda Burchell

Dear Histonetters,
  We are interested in morphometric analysis in lungs from rats who have an asthma like condition.  We are trying to look at ways to evaluate airway surface fluid and it's relation to airway size and airway degree of closure.  Is frozen sectioning the only way to handle the lungs without causing significant dehydration of the tissues?  How do we best fix these fluids?  Thanks in advance for your consideration of these questions!   Cindy B

LaCinda Burchell, BA, HT(ASCP)
University of Wisconsin-Madison, Medical School
Asthma and Allergy Research IHC Lab
600 Highland Ave.  CSC  K4/913
Madison, Wisconsin  53792

Phone: 608-262-3518
FAX:     608-263-3746

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