air-drying for SEM
I was planning to try a protocol for preparing tissue for SEM which called for
air-drying from freon-113 as the last step (ref: SEM, 1985, IV, pp. 1649-1664).
Unfortunately freon-113 no longer seems to be availible, and I guess is no
longer being manufactured. Would anyone know of, or be able to suggest a
substitute for this chemical. Thanks.
A. Thomas Read, PhD
Dept of Ophthalmology
Gage Institute of Research
University of Toronto
223 College St., Rm 308
Toronto, ON M5T 1R4 Canada
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