air-drying for SEM

From:tom.read@utoronto.ca

Hello,

I was planning to try a protocol for preparing tissue for SEM which called for 
air-drying from freon-113 as the last step (ref: SEM, 1985, IV, pp. 1649-1664). 
Unfortunately freon-113 no longer seems to be availible, and I guess is no 
longer being manufactured. Would anyone know of, or be able to suggest a 
substitute for this chemical. Thanks.



A. Thomas Read, PhD
Dept of Ophthalmology
Gage Institute of Research
University of Toronto
223 College St., Rm 308
Toronto, ON  M5T 1R4  Canada




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