Silver Degeneration Stain for CNS

From:"Jen, Shirley {Path~Palo Alto}"

Hi Everyone,
Does anyone know if there is any silver impregnation staining technique that
works on paraffin section to stain degenerated neuronal cells?  Or has
anybody have an experience in doing de Olmos' amino-cupric-silver stain and
does it work on paraffin section fixed in regular 10% formalin, instead of
perfusion with 4% paraformaldehyde and stained using free-floating frozen
section?  We would like to avoid de Olmos' technique, if possible.  Your
expertise is greatly appreciated.
Shirley Jen
Roche Bioscience
Palo Alto, CA 94304

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