Re: artifactual nuclear staining


is he doing HIER?  this can cause non-specific nuclear staining.
patsy wrote:

G'day folks - Have an email from a non-HistoNet buddy, who asks:

one of the post grads has a wee problem
he is using immunocytochemistry to localise some neural antigen (trysine
hydroxylase)(should present in the cytoplasm of his cells) he is using a
rabbit polyclonal followed by antirabbit biotin then avidin fluoroscein or
the problem is that all of the nuclei are staining - as if  you  used
propidium iodide - any suggestions - he has done a fair bit of immuno before

Well I dont know - - Any suggestions?
Bye now - Richard

Institute of Biomedical & Life Sciences, University of Glasgow
T direct 01796-474 480 --- E
"What should we expect? Everything."

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