From: | rueggp |
RichardWHorobin@aol.com wrote:
G'day folks - Have an email from a non-HistoNet buddy, who asks:one of the post grads has a wee problem
he is using immunocytochemistry to localise some neural antigen (trysine
hydroxylase)(should present in the cytoplasm of his cells) he is using a
rabbit polyclonal followed by antirabbit biotin then avidin fluoroscein or
Rhodamine.
the problem is that all of the nuclei are staining - as if you used
propidium iodide - any suggestions - he has done a fair bit of immuno beforeWell I dont know - - Any suggestions?
Bye now - RichardInstitute of Biomedical & Life Sciences, University of Glasgow
T direct 01796-474 480 --- E RichardWHorobin@aol.com
"What should we expect? Everything."