Re: UEA-1 Protocol

From:Barry Rittman

Usual to use 0.15M L-fucose in PBS as a competitive inhibitor .
other inhibitors are available but do not have that book at work.

Karen M Majahad wrote:

> I was wondering if anyone has a procedure for using the
> Vector Biotinylated Ulex Europaeus Agglutinin I on formalin
> fixed paraffin embedded sections that contain endothelial
> cells. A paper I read mentioned using L-Fucose as a negative
> control but did not explain in detail how to use the
> L-Fucose.Thanks in advance for any help.

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